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Insecticide Kit

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Several fatal infections like malaria, filariasis and dengue are transmitted by mosquitoes. The haphazard and extensive use of unusual insecticides in the public health programs as well as agricultural purposes for the control of insect's pests led to the development of resistance among various insects. Discovery of insecticide fight in insects is important from the point of view of insect control and can be through by either bioassay or biochemical methods. Bioassay is time uncontrollable and requires a large number of insects. Over and above this technique would not give any clue of the resistance mechanism, which is essential for use and selection of the right insecticide.
Test kit: Filter paper spot test method for organ phosphorus insecticide fight detection kit from single larva or adult mosquito based on biochemical mechanism has been urbanized. The kit is simple, fast and useful to use in the field. The kit contains material for 20 tests.

1. Test procedure - each larva or unfed adult mosquito collected from the field is independently homogenized in 0.5 ml buffer. Only 5 µl of homogenate is moved to substrate saturate filter paper. Once the filter paper is dried out, the paper is put into a petri-dish having freshly ready stain. The look of strong colour spot indicates growth of resistance against OP insecticide in mosquitoes.

2. Storage stability - it can be stored at room temperature and the storage space constancy of substrate impregnated filter paper was found to be more than 90 days as compared to shelf life of 15-30 days of any extra allied kind of paper developed somewhere else at refrigerated conditions.

3. Field trials - Extensive field trials conducted at diverse location indicate clear distinct colour spot between vulnerable and resistance mosquitoes. The evaluation of kit has been done at malaria research centre

Advantages: 1. Rapid as the kit takes only 15-30 minutes to get the result. 2. Small volume of only 2-5 µl of only mosquito homogenate is required when compared to big number of insects. 3. Staff and cost effective due to semi skilled individual can perform the test in the field itself and each test cost around only five rupees. The kit is light and weighs less too.